Article

Shorter unreported sequences in a RACE-Seq study involving seven tissues confirms ∼150 novel transcripts identified in MCF-7 cell line PacBio transcriptome, leaving ∼100 non-redundant transcripts exclusive to the cancer cell line

2017-01-30

Abstract excerpt

PacBio sequencing generates much longer reads compared to second-generation sequencing technologies, with a trade-off of lower throughput, higher error rate and more cost per base. The PacBio transcriptome of the breast cancer cell line MCF-7 was found to have ∼300 transcripts un-annotated in the current GENCODE (v25) or RefSeq, and missing in the liver, heart and brain PacBio transcriptomes [1]. RACE-sequencing (...

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Literature Corpus work: 0ed9b12d-6212-5e58-a87f-b73985bd1fc5
DOI: 10.1101/104257

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