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Article

Highly reliable creation of floxed alleles by electroporating single-cell embryos.

BMC biology - 4 Feb 2022

Sentmanat Monica F, White J Michael, Kouranova Evguenia, Cui Xiaoxia

Abstract excerpt

BACKGROUND: Floxed (flanked by loxP) alleles are a crucial portion of conditional knockout mouse models. However, an efficient and reliable strategy to flox genomic regions of any desired size is still lacking. RESULTS: Here, we demonstrate that the method combining electroporation of fertilized eggs with gRNA/Cas9 complexes and single-stranded oligodeoxynucleotides (ssODNs), assessing phasing of loxP insertions...

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Identifiers

PMID: 35115009
DOI: 10.1186/s12915-021-01223-w
PMCID: PMC8815186

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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