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Article

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice.

Journal of visualized experiments : JoVE - 24 Jun 2022

Tanimoto Yoko, Mikami Natsuki, Ishida Miyuki, Iki Natsumi, Kato Kanako, Sugiyama Fumihiro, Takahashi Satoru, Mizuno Seiya

Abstract excerpt

CRISPR-Cas technology has enabled the rapid and effortless generation of genetically modified mice. Specifically, mice and point mutant mice are readily produced by electroporation of CRISPR factors (and single-stranded oligo DNA donors) into the zygote. In contrast, gene cassette (>1 kb) knock-in and floxed mice are mainly generated by microinjection of CRISPR factors and double-stranded DNA donors into zygotes....

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Identifiers

PMID: 35815994
DOI: 10.3791/64161

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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