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Article

Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes.

Scientific reports - 21 Oct 2020

Alghadban Samy, Bouchareb Amine, Hinch Robert, Hernandez-Pliego Polinka, Biggs Daniel, Preece Chris, Davies Benjamin

Abstract excerpt

CRISPR/Cas9 machinery delivered as ribonucleoprotein (RNP) to the zygote has become a standard tool for the development of genetically modified mouse models. In recent years, a number of reports have demonstrated the effective delivery of CRISPR/Cas9 machinery via zygote electroporation as an alternative to the conventional delivery method of microinjection. In this study, we have performed side-by-side...

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Share to Clustered Regularly Interspaced Short Palindromic Repeats
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 33087834
DOI: 10.1038/s41598-020-74960-7
PMCID: PMC7578782

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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