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Accelerated embryonic stem cell screening with a highly efficient genotyping pipeline.

Molecular biology reports - 1 Apr 2022

Caothien Roger, Yu Charles, Tam Lucinda, Newman Robert, Nakao Brian, Alcantar Tuija, Bacarro Natasha, Reyes Juan, Pham Anna, Roose-Girma Merone

Abstract excerpt

INTRODUCTION: Gene targeting in mouse ES cells replaces or modifies genes of interest; conditional alleles, reporter knock-ins, and amino acid changes are common examples of how gene targeting is used. For example, enhanced green fluorescent protein or Cre recombinase is placed under the control of endogenous genes to define promoter expression patterns. METHODS AND RESULTS: The most important step in the process...

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PMID: 35107736
DOI: 10.1007/s11033-022-07165-y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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