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Article

An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.

Nucleic acids research - 1 Dec 2017

Fisher Cynthia L, Marks Hendrik, Cho Lily Ting-Yin, Andrews Robert, Wormald Sam, Carroll Thomas, Iyer Vivek, Tate Peri, Rosen Barry, Stunnenberg Hendrik G, Fisher Amanda G, Skarnes William C

Abstract excerpt

Mouse embryonic stem (ES) cells are a popular model system to study biological processes, though uncovering recessive phenotypes requires inactivating both alleles. Building upon resources from the International Knockout Mouse Consortium (IKMC), we developed a targeting vector for second allele inactivation in conditional-ready IKMC 'knockout-first' ES cell lines. We applied our technology to several epigenetic...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28981838
DOI: 10.1093/nar/gkx811
PMCID: PMC5716182

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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