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Article

A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells.

PloS one - 1 Jan 2016

Findlay Scott D, Vincent Krista M, Berman Jennifer R, Postovit Lynne-Marie

Abstract excerpt

The rapid adoption of gene editing tools such as CRISPRs and TALENs for research and eventually therapeutics necessitates assays that can rapidly detect and quantitate the desired alterations. Currently, the most commonly used assay employs "mismatch nucleases" T7E1 or "Surveyor" that recognize and cleave heteroduplexed DNA amplicons containing mismatched base-pairs. However, this assay is prone to false...

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Identifiers

PMID: 27089539
DOI: 10.1371/journal.pone.0153901
PMCID: PMC4835065

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SFRP1

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