Article
Direct MYD88L265P gene detection for diffuse large B-cell lymphoma (DLBCL) via a miniaturised CRISPR/dCas9-based sensing chip.
Lab on a chip - 15 Feb 2022
Sun Weihan, Guo Wei, Liu Zhiyi, Qiao Sennan, Wang Ziming, Wang Jiayu, Qu Lingxuan, Shan Liang, Sun Fei, Xu Shuping, Bai Ou, Liang Chongyang
Abstract excerpt
Traditional methods for single-nucleotide variants based on amplification and fluorescence signals require expensive reagents and cumbersome instruments, and they are time-consuming for each trial. Here, a porous anodised aluminium (PAA)-based sensing chip modified with deactivated Cas9 (dCas9) proteins and synthetic guide RNA (sgRNA) as the biorecognition receptor is developed, which can be used for the...
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