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Lanthanide Metal-Organic Framework-Integrated CRISPR-Cas Technology for Amplification-free Gene Mutation Assay.

Analytical chemistry - 19 Aug 2025

Liu Yongzhen, Yu Long, Gan Zhiwen, Feng Yumin, Tong Jiyu, Xiao Yuxiu

Abstract excerpt

Nucleic acid amplification remains a major bottleneck in CRISPR-based molecular diagnostics, limiting assay speed, simplicity, and accuracy. Herein, we report a lanthanide metal-organic framework (Ln-MOF)-integrated CRISPR-Cas12a platform for amplification-free detection of gene mutations with high sensitivity and specificity. In this system, target recognition activates Cas12a trans-cleavage, degrading a...

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PMID: 40769177
DOI: 10.1021/acs.analchem.5c01949

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