Article
Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy.
G3 (Bethesda, Md.) - 5 Jan 2017
Kane Nanci S, Vora Mehul, Varre Krishna J, Padgett Richard W
Abstract excerpt
Genome editing using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and associated nuclease (Cas9) enables specific genetic modifications, including deletions, insertions, and substitutions in numerous organisms, such as the fruit fly Drosophila melanogaster One challenge of the CRISPR/Cas9 system can be the laborious and time-consuming screening required to find CRISPR-induced...
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