Article
Screening for CRISPR/Cas9-induced mutations using a co-injection marker in the nematode Pristionchus pacificus.
Development genes and evolution - 1 May 2020
Nakayama Ken-Ichi, Ishita Yuuki, Chihara Takahiro, Okumura Misako
Abstract excerpt
CRISPR/Cas9 genome-editing methods are used to reveal functions of genes and molecular mechanisms underlying biological processes in many species, including nematodes. In evolutionary biology, the nematode Pristionchus pacificus is a satellite model and has been used to understand interesting phenomena such as phenotypic plasticity and self-recognition. In P. pacificus, CRISPR/Cas9-mediated mutations are induced...
Topics
- Animals
- CRISPR-Cas Systems
- Chemotaxis
- Electrophoresis, Microchip
- Gene Editing
- Genetic Markers
- Genome, Helminth
- Heterozygote
- Microinjections
- Models, Animal
- Mutation