Article
Rational Regulation of Reaction Specificity of Nitrilase for Efficient Biosynthesis of 2-Chloronicotinic Acid through a Single Site Mutation.
Applied and environmental microbiology - 8 Mar 2022
Dai An-Di, Tang Xiao-Ling, Wu Zhe-Ming, Tang Jiang-Tao, Zheng Ren-Chao, Zheng Yu-Guo
Abstract excerpt
Nitrilase-catalyzed hydrolysis of 2-chloronicotinonitrile (2-CN) is a promising approach for the efficient synthesis of 2-chloronicotinic acid (2-CA). The development of nitrilase with ideal catalytic properties is crucial for the biosynthetic route with industrial potential. Herein, a nitrilase from Rhodococcus zopfii (RzNIT), which showed much higher hydration activity than hydrolysis activity, was designed for...
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