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Article

Reprogramming regioselectivity of nitrile hydratase towards adiponitrile via semi-rational design of a key "switch" residue.

International journal of biological macromolecules - 1 Apr 2026

Guo Yi, Song Yingjie, Chen Guobing, Zhang Hua, Li Jienan, Wang Li, Liang Changhai

Abstract excerpt

5-Cyanovaleramide (5-CVAM) is a monocyanoamide intermediate used for synthesizing herbicides and fine chemicals. Compared to chemical catalysis, nitrile hydratase (NHase, EC 4.2.1.84) is a green catalyst for the conversion of nitrile to amide. In our previous work, we heterologously expressed the NHase gene from Rhodococcus erythropolis CCM2595 (ReNHase) in Escherichia coli (E.coli) by optimizing the ribosomal...

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PMID: 41865929
DOI: 10.1016/j.ijbiomac.2026.151582

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ADM

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