Article
Computational design of new enzymes for hydrolysis and synthesis of third-generation cephalosporin antibiotics.
Enzyme and microbial technology - 1 Oct 2020
Xue Jing, Wang Pengyu, Kuang Jianyong, Zhu Yushan
Abstract excerpt
Engineering active sites in inert scaffolds to catalyze chemical transformations with unnatural substrates is still a great challenge for enzyme catalysis. In this research, a p-nitrobenzyl esterase from Bacillus subtilis was identified from the structural database, and a double mutant E115A/E188A was designed to afford catalytic activities toward the hydrolysis of ceftizoxime. A quadruple mutant...
Topics
- Anti-Bacterial Agents
- Bacillus subtilis
- Carboxylic Ester Hydrolases
- Catalysis
- Catalytic Domain
- Ceftizoxime
- Cephalosporins
- Hydrolysis
- Kinetics
- Models, Molecular
- Mutation