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Computational design of new enzymes for hydrolysis and synthesis of third-generation cephalosporin antibiotics.

Enzyme and microbial technology - 1 Oct 2020

Xue Jing, Wang Pengyu, Kuang Jianyong, Zhu Yushan

Abstract excerpt

Engineering active sites in inert scaffolds to catalyze chemical transformations with unnatural substrates is still a great challenge for enzyme catalysis. In this research, a p-nitrobenzyl esterase from Bacillus subtilis was identified from the structural database, and a double mutant E115A/E188A was designed to afford catalytic activities toward the hydrolysis of ceftizoxime. A quadruple mutant...

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Identifiers

PMID: 32912699
DOI: 10.1016/j.enzmictec.2020.109649

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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