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Article

Propagation of SARS-CoV-2 in Calu-3 Cells to Eliminate Mutations in the Furin Cleavage Site of Spike.

Viruses - 4 Dec 2021

Baczenas John James, Andersen Hanne, Rashid Sujatha, Yarmosh David, Puthuveetil Nikhita, Parker Michael, Bradford Rebecca, Florence Clint, Stemple Kimberly J, Lewis Mark G, O'Connor Shelby L

Abstract excerpt

SARS-CoV-2 pathogenesis, vaccine, and therapeutic studies rely on the use of animals challenged with highly pathogenic virus stocks produced in cell cultures. Ideally, these virus stocks should be genetically and functionally similar to the original clinical isolate, retaining wild-type properties to be reliably used in animal model studies. It is well-established that SARS-CoV-2 isolates serially passaged on...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 34960703
DOI: 10.3390/v13122434
PMCID: PMC8704555

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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