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A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells

17 Jun 2021

Abstract excerpt

An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially...

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PMID: 34140522
DOI: 10.1038/s41541-021-00346-z

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