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Article

Generating Zebrafish RNA-Less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022

Kumari Priyanka, Sturgeon Morgan, Bonde Gregory, Cornell Robert A

Abstract excerpt

Danio rerio (zebrafish), traditionally used in forward genetic screens, has in the last decade become a popular model for reverse genetic studies with the introduction of TALENS, zinc finger nucleases, and CRISPR/Cas9. Unexpectedly, homozygous frameshift mutations generated by these tools frequently result in phenotypes that are less penetrant than those seen in embryos injected with antisense morpholino...

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PMID: 34913119
DOI: 10.1007/978-1-0716-1847-9_8
PMCID: PMC10136374

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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