Article
A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish.
Disease models & mechanisms - 1 Jun 2017
Prykhozhij Sergey V, Steele Shelby L, Razaghi Babak, Berman Jason N
Abstract excerpt
Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (Danio rerio) to generate frameshift mutations in protein-coding genes. Although there are methods to detect, quantify and sequence CRISPR/Cas9-induced mutations, identifying mutations in F1 heterozygous fish remains challenging....
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