Article
Grouped-seq for integrated phenotypic and transcriptomic screening of patient-derived tumor organoids.
Nucleic acids research - 21 Mar 2022
Wu Yushuai, Li Kaiyi, Li Yaqian, Sun Tao, Liu Chang, Dong Chunhui, Zhao Tian, Tang Decong, Chen Xiaojie, Chen Xiaofang, Liu Peng
Abstract excerpt
Patient-derived tumor organoids (PDOs) have emerged as a reliable in vitro model for drug discovery. However, RNA sequencing-based analysis of PDOs treated with drugs has not been realized in a high-throughput format due to the limited quantity of organoids. Here, we translated a newly developed pooled RNA-seq methodology onto a superhydrophobic microwell array chip to realize an assay of genome-wide RNA output...
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