Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Monitoring the binding and insertion of a single transmembrane protein by an insertase.

Nature communications - 6 Dec 2021

Laskowski Pawel R, Pluhackova Kristyna, Haase Maximilian, Lang Brian M, Nagler Gisela, Kuhn Andreas, Müller Daniel J

Abstract excerpt

Cells employ highly conserved families of insertases and translocases to insert and fold proteins into membranes. How insertases insert and fold membrane proteins is not fully known. To investigate how the bacterial insertase YidC facilitates this process, we here combine single-molecule force spectroscopy and fluorescence spectroscopy approaches, and molecular dynamics simulations. We observe that within 2 ms,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Membrane
Share to Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Membrane Transport Proteins

Share to Microscopy, Atomic Force

Share to Microscopy, Confocal

Share to Molecular Dynamics Simulation

Share to Protein Conformation, alpha-Helical

Share to Spectrometry, Fluorescence

Source

Open in PubMed Open full text

Identifiers

PMID: 34873152
DOI: 10.1038/s41467-021-27315-3
PMCID: PMC8648943

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Monitoring the binding and insertion of a single transmembrane protein by an insertase.PMID 34873152Read ↗

Tracking the Stepwise Movement of a Membrane-inserting Protein In Vivo.Semantic proximity Read ↗

Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC.Semantic proximity Read ↗

Substrate-dependent conformational dynamics of the Escherichia coli membrane insertase YidC.Semantic proximity Read ↗

Structure of a bacterial type III secretion system in contact with a host membrane in situSemantic proximity Read ↗

Free-energy landscapes of membrane co-translocational protein unfolding.Semantic proximity Read ↗

Dynamic disulfide scanning of the membrane-inserting Pf3 coat protein reveals multiple YidC substrate contacts.Semantic proximity Read ↗

Deciphering the structural intricacy in virulence effectors for proton-motive force mediated unfolding and type-III protein secretionSemantic proximity Read ↗

The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis.Semantic proximity Read ↗

Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC.Semantic proximity Read ↗

A structural model of flagellar filament switching across multiple bacterial species.Semantic proximity Read ↗

Correlating membrane-protein dynamics with function: Integrating bioinformatics, molecular dynamics, and single-molecule FRETSemantic proximity Read ↗

Multistep protein unfolding during nanopore translocation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.