Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC.

doi:10.1371/journal.pone.0059023

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Article

Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC.

PloS one - 1 Jan 2013

Winterfeld Sophie, Ernst Stefan, Börsch Michael, Gerken Uwe, Kuhn Andreas

Abstract excerpt

Membrane protein translocation and insertion is a central issue in biology. Here we focus on a minimal system, the membrane insertase YidC of Escherichia coli that inserts small proteins into the cytoplasmic membrane. In a reconstituted system individual insertion processes were followed by single-pair fluorescence resonance energy transfer (FRET), with a pair of fluorophores on YidC and the substrate Pf3 coat...

Topics

Escherichia coli
Escherichia coli Proteins
Fluorescence Resonance Energy Transfer
Membrane Proteins
Membrane Transport Proteins
Microscopy, Confocal
Mutation
Protein Binding

Source

Open in PubMed Open full text

Identifiers

PMID: 23527078
DOI: 10.1371/journal.pone.0059023
PMCID: PMC3602594

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