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Article

Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC.

PloS one - 1 Jan 2013

Winterfeld Sophie, Ernst Stefan, Börsch Michael, Gerken Uwe, Kuhn Andreas

Abstract excerpt

Membrane protein translocation and insertion is a central issue in biology. Here we focus on a minimal system, the membrane insertase YidC of Escherichia coli that inserts small proteins into the cytoplasmic membrane. In a reconstituted system individual insertion processes were followed by single-pair fluorescence resonance energy transfer (FRET), with a pair of fluorophores on YidC and the substrate Pf3 coat...

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Identifiers

PMID: 23527078
DOI: 10.1371/journal.pone.0059023
PMCID: PMC3602594

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC.PMID 23527078Read ↗

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