Article
The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis.
FEBS letters - 10 Dec 2008
Klenner Christian, Yuan Jijun, Dalbey Ross E, Kuhn Andreas
Abstract excerpt
The coat protein of bacteriophage Pf3 is inserted into the plasma membrane of Escherichia coli by the insertase YidC. To identify which of the six transmembrane regions of YidC bind the single-spanning Pf3 coat protein during membrane protein biogenesis, we used the disulfide cross-linking approach. We generated single cysteines in each of the transmembrane regions of YidC and in the center of the hydrophobic...
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