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Article

Gene editing enables rapid engineering of complex antibiotic assembly lines.

Nature communications - 25 Nov 2021

Thong Wei Li, Zhang Yingxin, Zhuo Ying, Robins Katherine J, Fyans Joanna K, Herbert Abigail J, Law Brian J C, Micklefield Jason

Abstract excerpt

Re-engineering biosynthetic assembly lines, including nonribosomal peptide synthetases (NRPS) and related megasynthase enzymes, is a powerful route to new antibiotics and other bioactive natural products that are too complex for chemical synthesis. However, engineering megasynthases is very challenging using current methods. Here, we describe how CRISPR-Cas9 gene editing can be exploited to rapidly engineer one...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 34824225
DOI: 10.1038/s41467-021-27139-1
PMCID: PMC8616955

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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