Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

21 Jan 2014

Abstract excerpt

Recent developments in next-generation sequencing technologies have brought recognition of microbial genomes as a rich resource for novel natural product discovery. However, owing to the scarcity of efficient procedures to connect genes to molecules, only a small fraction of secondary metabolomes have been investigated to date. Transformation-associated recombination (TAR) cloning takes advantage of the natural...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biochemical and Structural Characterization
Share to Microbial Natural Products and Biosynthesis
Share to Plant biochemistry and biosynthesis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 24449899
DOI: 10.1073/pnas.1319584111

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin APMID 24449899Read ↗

Atolypenes, Tricyclic Bacterial Sesterterpenes Discovered Using a Multiplexed In Vitro Cas9-TAR Gene Cluster Refactoring ApproachSemantic proximity Read ↗

mpCRISTAR: Multiple Plasmid Approach for CRISPR/Cas9 and TAR-Mediated Multiplexed Refactoring of Natural Product Biosynthetic Gene Clusters.Semantic proximity Read ↗

Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology.Semantic proximity Read ↗

A guide to genome mining and genetic manipulation of biosynthetic gene clusters in StreptomycesSemantic proximity Read ↗

Versatile filamentous fungal host highly-producing heterologous natural products developed by genome editing-mediated engineering of multiple metabolic pathwaysSemantic proximity Read ↗

Improvement of A21978C production in Streptomyces roseosporus by reporter-guided rpsL mutation selection.Semantic proximity Read ↗

Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome EditingSemantic proximity Read ↗

Genetic platforms for heterologous expression of microbial natural productsSemantic proximity Read ↗

Targeted activation of silent natural product biosynthesis pathways by reporter-guided mutant selection.Semantic proximity Read ↗

A highly efficient Cre-based Clostridial workflow for genomic integration and expression of large biosynthetic pathwaysSemantic proximity Read ↗

Gene editing enables rapid engineering of complex antibiotic assembly lines.Semantic proximity Read ↗

High-throughput engineering and modification of non-ribosomal peptide synthetases based on Golden Gate assemblySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.