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Article

Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes.

Proceedings of the National Academy of Sciences of the United States of America - 17 Jul 2007

Fischbach Michael A, Lai Jonathan R, Roche Eric D, Walsh Christopher T, Liu David R

Abstract excerpt

Nonribosomal peptides (NRPs) are produced by NRP synthetase (NRPS) enzymes that function as molecular assembly lines. The modular architecture of NRPSs suggests that a domain responsible for activating a building block could be replaced with a domain from a foreign NRPS to create a chimeric assem...

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Identifiers

PMID: 17620609
DOI: 10.1073/pnas.0705348104
PMCID: PMC1924594

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17620609-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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