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Article

Probing the stability of the SpCas9-DNA complex after cleavage.

Nucleic acids research - 2 Dec 2021

Aldag Pierre, Welzel Fabian, Jakob Leonhard, Schmidbauer Andreas, Rutkauskas Marius, Fettes Fergus, Grohmann Dina, Seidel Ralf

Abstract excerpt

CRISPR-Cas9 is a ribonucleoprotein complex that sequence-specifically binds and cleaves double-stranded DNA. Wildtype Cas9 and its nickase and cleavage-incompetent mutants have been used in various biological techniques due to their versatility and programmable specificity. Cas9 has been shown to bind very stably to DNA even after cleavage of the individual DNA strands, inhibiting further turnovers and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34792162
DOI: 10.1093/nar/gkab1072
PMCID: PMC8643700

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