Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

Proceedings of the National Academy of Sciences of the United States of America - 8 Jul 2014

Szczelkun Mark D, Tikhomirova Maria S, Sinkunas Tomas, Gasiunas Giedrius, Karvelis Tautvydas, Pschera Patrizia, Siksnys Virginijus, Seidel Ralf

Abstract excerpt

Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems protect bacteria and archaea from infection by viruses and plasmids. Central to this defense is a ribonucleoprotein complex that produces RNA-guided cleavage of foreign nucleic acids. In DNA-targe...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Associated Proteins
Share to DNA, Superhelical

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to PAM gene

Share to Mutation

Share to Protein Conformation

Share to RNA, Small Untranslated

Source

Open in PubMed Open full text

Identifiers

PMID: 24912165
DOI: 10.1073/pnas.1402597111
PMCID: PMC4103346

Genes

PAM

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-24912165-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.PMID 24912165Read ↗

Probing the stability of the SpCas9-DNA complex after cleavage.Semantic proximity Read ↗

Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence.Semantic proximity Read ↗

CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment.Semantic proximity Read ↗

The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activitySemantic proximity Read ↗

Protein-Mutation-Induced Conformational Changes of the DNA and Nuclease Domain in CRISPR/Cas9 Systems by Molecular Dynamics Simulations.Semantic proximity Read ↗

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3Semantic proximity Read ↗

Two distinct DNA binding modes guide dual roles of a CRISPR-Cas protein complex.Semantic proximity Read ↗

Differential Allosteric Modulation of Cas9 Specificity.Semantic proximity Read ↗

Structural basis for self-cleavage prevention by tag:anti-tag pairing complementarity in type VI Cas13 CRISPR systems.Semantic proximity Read ↗

Unveiling the RNA-mediated allosteric activation discloses functional hotspots in CRISPR-Cas13a.Semantic proximity Read ↗

Conformational Dynamics of DNA Binding and Cas3 Recruitment by the CRISPR RNA-Guided Cascade Complex.Semantic proximity Read ↗

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.