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Article

Engineering of the genome editing protein Cas9 to slide along DNA.

Scientific reports - 8 Jul 2021

Banerjee Trishit, Takahashi Hiroto, Subekti Dwiky Rendra Graha, Kamagata Kiyoto

Abstract excerpt

The genome editing protein Cas9 faces engineering challenges in improving off-target DNA cleavage and low editing efficiency. In this study, we aimed to engineer Cas9 to be able to slide along DNA, which might facilitate genome editing and reduce off-target cleavage. We used two approaches to achieve this: reducing the sliding friction along DNA by removing the interactions of Cas9 residues with DNA and...

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Identifiers

PMID: 34239016
DOI: 10.1038/s41598-021-93685-9
PMCID: PMC8266852

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