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PlmCas12e Utilizes Glu662 to Prevent Cleavage Site Occupation by Positively Charged Residues Before Target Strand Cleavage.

Molecules (Basel, Switzerland) - 25 Oct 2024

Liu Jinchu, Zhu Lizhe

Abstract excerpt

CRISPR-Cas12e is a recently identified gene-editing tool mainly known because its relatively small size benefits cell delivery. Drastically different from Cas9, it creates a blunt-end double-strand breakage of the DNA via two cleavage sites; Cas12e produces a sticky-end double-strand breakage of the DNA through only one cleavage site in its RuvC domain, meaning two consecutive cleavage events first on the...

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PMID: 39519677
DOI: 10.3390/molecules29215036
PMCID: PMC11547573

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