Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning.

STAR protocols - 17 Dec 2021

Haas Cyrus M, Francino-Urdaniz Irene M, Steiner Paul J, Whitehead Timothy A

Abstract excerpt

Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all potential single S RBD escape mutants can be identified for monoclonal antibodies that directly compete with angiotensin-converting enzyme 2 for binding. Six to 10 antibodies...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Angiotensin-Converting Enzyme 2
Share to Binding Sites

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to COVID-19

Share to DNA Mutational Analysis

Share to Mutation

Share to Saccharomyces cerevisiae

Share to SARS-CoV-2

Share to Spike Glycoprotein, Coronavirus

Source

Open in PubMed Open full text

Identifiers

PMID: 34568839
DOI: 10.1016/j.xpro.2021.100869
PMCID: PMC8455247

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning.PMID 34568839Read ↗

One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screeningSemantic proximity Read ↗

One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screeningSemantic proximity Read ↗

An antibody-escape estimator for mutations to the SARS-CoV-2 receptor-binding domainSemantic proximity Read ↗

Rapidly Adaptable Multiplexed Yeast Surface Display Serological Assay for Immune Escape Screening of SARS-CoV-2 VariantsSemantic proximity Read ↗

High-throughput screening of mutations affecting SARS-CoV-2 spike functions.Semantic proximity Read ↗

Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodiesSemantic proximity Read ↗

Mutational escape from the polyclonal antibody response to SARS-CoV-2 infection is largely shaped by a single class of antibodiesSemantic proximity Read ↗

Susceptibility of broad reactivity nanobodies to resistance mutations in the S2 domain of SARS-CoV-2 predicted by yeast display deep mutational scanning.Semantic proximity Read ↗

Mapping mutations to the SARS-CoV-2 RBD that escape binding by different classes of antibodies.Semantic proximity Read ↗

Dissecting serum polyclonal antibody escape to SARS-CoV-2 variants by deep mutational learningSemantic proximity Read ↗

Dissecting serum polyclonal antibody escape to SARS-CoV-2 variants by deep mutational learning.Semantic proximity Read ↗

Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.