Article
Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a non-cancer human cell line.
PloS one - 1 Jan 2021
Kim Daria V, Kulishova Liliya M, Torgasheva Natalia A, Melentyev Vasily S, Dianov Grigory L, Medvedev Sergey P, Zakian Suren M, Zharkov Dmitry O
Abstract excerpt
The major human apurinic/apyrimidinic (AP) site endonuclease, APEX1, is a central player in the base excision DNA repair (BER) pathway and has a role in the regulation of DNA binding by transcription factors. In vertebrates, APEX1 knockouts are embryonic lethal, and only a handful of knockout cell lines are known. To facilitate studies of multiple functions of this protein in human cells, we have used the...
Topics
- CRISPR-Cas Systems
- Cell Cycle Checkpoints
- DNA Repair
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Gene Editing
- HEK293 Cells
- Humans
- Hydrogen Peroxide
- Methyl Methanesulfonate
- Phenotype
- RNA, Guide, CRISPR-Cas Systems