Article
Designer Nuclease-Mediated Generation of Knockout THP1 Cells.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Schmidt Tobias, Schmid-Burgk Jonathan L, Ebert Thomas S, Gaidt Moritz M, Hornung Veit
Abstract excerpt
Recent developments in the field of designer nucleases allow the efficient and specific manipulation of genomic architectures in eukaryotic cell lines. To this end, it has become possible to introduce DNA double strand breaks (DSBs) at user-defined genomic loci. If located in critical coding regions of genes, thus induced DSBs can lead to insertions or deletions (indels) that result in frameshift mutations and...
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