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Article

A precisely adjustable, variation-suppressed eukaryotic transcriptional controller to enable genetic discovery.

eLife - 3 Aug 2021

Azizoglu Asli, Brent Roger, Rudolf Fabian

Abstract excerpt

Conditional expression of genes and observation of phenotype remain central to biological discovery. Current methods enable either on/off or imprecisely controlled graded gene expression. We developed a 'well-tempered' controller, WTC846, for precisely adjustable, graded, growth condition independent expression of genes in Saccharomyces cerevisiae. Controlled genes are expressed from a strong semisynthetic...

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Identifiers

PMID: 34342575
DOI: 10.7554/eLife.69549
PMCID: PMC8421071

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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