Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae.

Abstract excerpt

The strong overexpression or complete deletion of a gene gives only limited information about its control over a certain phenotype or pathway. Gene function studies based on these methods are therefore incomplete. To effect facile manipulation of gene expression across a full continuum of possible expression levels, we recently created a library of mutant promoters. Here, we provide the detailed characterization...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Gene Expression Regulation, Fungal