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Article

Prediction of the sequence-specific cleavage activity of Cas9 variants.

Nature biotechnology - 1 Nov 2020

Kim Nahye, Kim Hui Kwon, Lee Sungtae, Seo Jung Hwa, Choi Jae Woo, Park Jinman, Min Seonwoo, Yoon Sungroh, Cho Sung-Rae, Kim Hyongbum Henry

Abstract excerpt

Several Streptococcus pyogenes Cas9 (SpCas9) variants have been developed to improve an enzyme's specificity or to alter or broaden its protospacer-adjacent motif (PAM) compatibility, but selecting the optimal variant for a given target sequence and application remains difficult. To build computational models to predict the sequence-specific activity of 13 SpCas9 variants, we first assessed their cleavage...

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PMID: 32514125
DOI: 10.1038/s41587-020-0537-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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