Ultrasensitive detection of gene-PIK3CAH1047R mutation based on cascaded strand displacement amplification and trans-cleavage ability of CRISPR/Cas12a.

doi:10.1016/j.talanta.2021.122415

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Article

Ultrasensitive detection of gene-PIK3CAH1047R mutation based on cascaded strand displacement amplification and trans-cleavage ability of CRISPR/Cas12a.

Talanta - 1 Sept 2021

Deng Yuanyi, Cao Gaihua, Chen Xiaolong, Yang Mei, Huo Danqun, Hou Changjun

Abstract excerpt

Low abundance gene-PIK3CAH1047R mutation detection is crucial for the clinical diagnosis and treatment of breast cancer. Here, a fluorescent biosensor which combines cascaded strand displacement amplification (C-SDA) and trans-cleavage ability of CRISPR/Cas12a was established to ultra-sensitively...

Topics

Biosensing Techniques
CRISPR-Cas Systems
Class I Phosphatidylinositol 3-Kinases
Clustered Regularly Interspaced Short Palindromic Repeats
Humans
Mutation

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