Article
CRISPR/Cas12a-based biosensing platform for precise and efficient screening of CRISPR/Cas9-induced biallelic mutants.
Talanta - 1 Apr 2020
Xiao Guohui, Liu Shuyan, Liu Houming, He Xing, Zhang Su, Liang Zhihang, Guo Huixin, Ou Min, Zhou Lin, Liu Lei, Zhang Tianyu, Zhang Guoliang
Abstract excerpt
CRISPR/Cas9 is a robust tool to manipulate genes in a wide range of species. Although several methods are introduced to identify the CRISPR/Cas9-induced mutations, they are labor-intensive, costly, and not easy to use or were sequence-limited. Moreover, few of them could identify the biallelic mutants that are the desired outcomes of targeted mutagenesis. Recently, a CRISPR/Cas12a-mediated biosensing platform was...
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