Article
CRISPR/Cas12a-based biosensing platform for precise and efficient screening of CRISPR/Cas9-induced biallelic mutants.
Talanta - 1 Apr 2020
Xiao Guohui, Liu Shuyan, Liu Houming, He Xing, Zhang Su, Liang Zhihang, Guo Huixin, Ou Min, Zhou Lin, Liu Lei, Zhang Tianyu, Zhang Guoliang
Abstract excerpt
CRISPR/Cas9 is a robust tool to manipulate genes in a wide range of species. Although several methods are introduced to identify the CRISPR/Cas9-induced mutations, they are labor-intensive, costly, and not easy to use or were sequence-limited. Moreover, few of them could identify the biallelic mu...
Topics
- Biosensing Techniques
- CRISPR-Cas Systems
- Humans
- Mutation
- RNA
- THP-1 Cells