Article
Single-nucleotide variant of PIK3CA H1047R gene assay by CRISPR/Cas12a combined with rolling circle amplification.
Analytica chimica acta - 16 Oct 2021
Cao Gaihua, Chen Xiaolong, Deng Yuanyi, Nie Fuping, Liu Yin, Wang Guoming, Huo Danqun, Hou Changjun
Abstract excerpt
PIK3CA H1047R gene plays an important role in the PI3K/Akt/mTOR signaling pathway, and its mutation is closely related to the occurrence and development of breast cancer and Lipoblastoma. Therefore, it is of great value to detect the PIK3CA H1047R mutant gene. Here, an analytical method coupled CRISPR/Cas12a with rolling circle amplification (RCA) technology was constructed for ultra-sensitive and specific...
Topics
- CRISPR-Cas Systems
- Class I Phosphatidylinositol 3-Kinases
- Humans
- Mutation
- Nucleotides
- Phosphatidylinositol 3-Kinases