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Article

Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster.

PLoS genetics - 1 May 2021

Stevens Leslie M, Kim Goheun, Koromila Theodora, Steele John W, McGehee James, Stathopoulos Angelike, Stein David S

Abstract excerpt

Here we describe the development and characterization of the photo-N-degron, a peptide tag that can be used in optogenetic studies of protein function in vivo. The photo-N-degron can be expressed as a genetic fusion to the amino termini of other proteins, where it undergoes a blue light-dependent conformational change that exposes a signal for the class of ubiquitin ligases, the N-recognins, which mediate the...

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PMID: 33999957
DOI: 10.1371/journal.pgen.1009544
PMCID: PMC8158876

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