Article
A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans.
Development (Cambridge, England) - 15 Jul 2017
Wang Shaohe, Tang Ngang Heok, Lara-Gonzalez Pablo, Zhao Zhiling, Cheerambathur Dhanya K, Prevo Bram, Chisholm Andrew D, Desai Arshad, Oegema Karen
Abstract excerpt
Proteins that are essential for embryo production, cell division and early embryonic events are frequently reused later in embryogenesis, during organismal development or in the adult. Examining protein function across these different biological contexts requires tissue-specific perturbation. Here, we describe a method that uses expression of a fusion between a GFP-targeting nanobody and a SOCS-box containing...
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