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Article

The N-degron approach to create temperature-sensitive mutants in Schizosaccharomyces pombe.

Methods (San Diego, Calif.) - 1 Jul 2004

Rajagopalan Srividya, Liling Zheng, Liu Jianhua, Balasubramanian Mohan

Abstract excerpt

Conditional mutants are a vital tool for analysis of gene function. The use of temperature-sensitive mutants in Schizosaccharomyces pombe has significantly promoted understanding of many cellular processes. A portable heat-inducible amino-terminal degron (N-degron) for conditional degradation of a gene product has been previously described in Saccharomyces cerevisiae. This paper describes the adaptation of the...

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Identifiers

PMID: 15157887
DOI: 10.1016/j.ymeth.2003.11.015

Genes

DHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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