Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant.

Abstract excerpt

Thermophilic organisms are extensively studied in industrial biotechnology, for exploration of the limits of life, and in other contexts. Their optimal growth at high temperatures presents a challenge for the development of genetic tools for their genome editing, since genetic markers and selection substrates are often thermolabile. We sought to develop a thermostable CRISPR-Cas9 based system for genome editing...

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