Article

A thermostable Cas9 with increased lifetime in human plasma

2017-05-16

Abstract excerpt

CRISPR-Cas9 is a powerful technology that has enabled genome editing in a wide range of species. However, the currently developed Cas9 homologs all originate from mesophilic bacteria, making them susceptible to proteolytic degradation and unsuitable for applications requiring function at elevated temperatures. Here, we show that the Cas9 protein from the thermophilic bacterium Geobacillus stearothermophilus (GeoC...

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Literature Corpus work: 190a2738-97f6-5a5e-bf0c-9468cd2a6c4d
DOI: 10.1101/138867

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