Article
Creating RNA Specific C-to-U Editase from APOBEC3A by Separation of Its Activities on DNA and RNA Substrates.
ACS synthetic biology - 21 May 2021
Tang Guiyue, Xie Bingran, Hong Xiangna, Qin Hang, Wang Jingfang, Huang Hai, Hao Pei, Li Xuan
Abstract excerpt
APOBEC3A (A3A) is a cytidine deaminase involved in innate immune response and is able to catalyze deamination on both DNA and RNA substrates. It was used in creating the CRISPR-mediated base editor, but has since been held back due to its dual activities. On the other hand, it has been a challenge to separate A3A's dual activities in order to enable it for single-base RNA editors. Here we developed the reporter...
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