Article
A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor.
Genome research - 1 May 2021
Lu Hengxing, Liu Jun, Feng Tao, Guo Zihang, Yin Yunjun, Gao Fei, Cao Gengsheng, Du Xuguang, Wu Sen
Abstract excerpt
Targeted mutagenesis in model organisms is key for gene functional annotation and biomedical research. Despite technological advances in gene editing by the CRISPR-Cas9 systems, rapid and efficient introduction of site-directed mutations remains a challenge in large animal models. Here, we developed a robust and flexible insertional mutagenesis strategy, homology-independent targeted trapping (HIT-trapping),...
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