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Article

Accurate SNV detection in single cells by transposon-based whole-genome amplification of complementary strands.

Proceedings of the National Academy of Sciences of the United States of America - 23 Feb 2021

Xing Dong, Tan Longzhi, Chang Chi-Han, Li Heng, Xie X Sunney

Abstract excerpt

Single-nucleotide variants (SNVs), pertinent to aging and disease, occur sporadically in the human genome, hence necessitating single-cell measurements. However, detection of single-cell SNVs suffers from false positives (FPs) due to intracellular single-stranded DNA damage and the process of whole-genome amplification (WGA). Here, we report a single-cell WGA method termed multiplexed end-tagging amplification of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33593904
DOI: 10.1073/pnas.2013106118
PMCID: PMC7923680

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