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SMOOTH-seq: single-cell genome sequencing of human cells on a third-generation sequencing platform.

Genome biology - 30 Jun 2021

Fan Xiaoying, Yang Cheng, Li Wen, Bai Xiuzhen, Zhou Xin, Xie Haoling, Wen Lu, Tang Fuchou

Abstract excerpt

There is no effective way to detect structure variations (SVs) and extra-chromosomal circular DNAs (ecDNAs) at single-cell whole-genome level. Here, we develop a novel third-generation sequencing platform-based single-cell whole-genome sequencing (scWGS) method named SMOOTH-seq (single-molecule real-time sequencing of long fragments amplified through transposon insertion). We evaluate the method for detecting...

Topics

Base Sequence
Chromosome Mapping
Colorectal Neoplasms
DNA Copy Number Variations
DNA Transposable Elements
DNA, Circular
Genome, Human
HEK293 Cells
High-Throughput Nucleotide Sequencing
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

K562 Cells

Polymorphism, Single Nucleotide

Single-Cell Analysis

Whole Genome Sequencing

Source

Open in PubMed Open full text

Identifiers

PMID: 34193237
DOI: 10.1186/s13059-021-02406-y
PMCID: PMC8247186

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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