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Article

Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency.

Nucleic acids research - 18 Mar 2021

Li Donghui, Sun Xiaoji, Yu Fangzhou, Perle Mary Ann, Araten David, Boeke Jef D

Abstract excerpt

The CRISPR/Cas9 system is a technology for genome engineering, which has been applied to indel mutations in genes as well as targeted gene deletion and replacement. Here, we describe paired gRNA deletions along the PIGA locus on the human X chromosome ranging from 17 kb to 2 Mb. We found no compelling linear correlation between deletion size and the deletion efficiency, and there is no substantial impact of...

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Identifiers

PMID: 33591308
DOI: 10.1093/nar/gkab035
PMCID: PMC7969003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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