Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Flow cytometry-based quantification of targeted knock-in events in human cell lines using a GPI-anchor biosynthesis gene PIGP.

Bioscience reports - 22 Dec 2021

Rahman Md Lutfur, Hyodo Toshinori, Hasan Muhammad Nazmul, Mihara Yuko, Karnan Sivasundaram, Ota Akinobu, Tsuzuki Shinobu, Hosokawa Yoshitaka, Konishi Hiroyuki

Abstract excerpt

Targeted knock-in supported by the CRISPR/Cas systems enables the insertion, deletion, and substitution of genome sequences exactly as designed. Although this technology is considered to have wide range of applications in life sciences, one of its prerequisites for practical use is to improve the efficiency, precision, and specificity achieved. To improve the efficiency of targeted knock-in, there first needs to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Membrane
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Cas Systems

Share to Gene Knock-In Techniques

Share to Genes, Reporter

Share to GPI-Linked Proteins

Share to HCT116 Cells

Share to Hexosyltransferases

Source

Open in PubMed Open full text

Identifiers

PMID: 34750615
DOI: 10.1042/BSR20212231
PMCID: PMC8661509

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Flow cytometry-based quantification of targeted knock-in events in human cell lines using a GPI-anchor biosynthesis gene PIGP.PMID 34750615Read ↗

Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency.Semantic proximity Read ↗

Bi-allelic Variants in the GPI Transamidase Subunit PIGK Cause a Neurodevelopmental Syndrome with Hypotonia, Cerebellar Atrophy, and Epilepsy.Semantic proximity Read ↗

A CRISPR-Cas9-engineered mouse model for GPI-anchor deficiency mirrors human phenotypes and exhibits hippocampal synaptic dysfunctions.Semantic proximity Read ↗

Hypomorphic mutations in PGAP2, encoding a GPI-anchor-remodeling protein, cause autosomal-recessive intellectual disability.Semantic proximity Read ↗

A CRISPR-Cas9–engineered mouse model for GPI-anchor deficiency mirrors human phenotypes and exhibits hippocampal synaptic dysfunctionsSemantic proximity Read ↗

Genetic Dissection of the role of <i>Piga</i> and <i>Pgap2</i> in the embryonic mouse brainSemantic proximity Read ↗

Mutations in PIGY: expanding the phenotype of inherited glycosylphosphatidylinositol deficiencies.Semantic proximity Read ↗

The GPI-anchor biosynthesis pathway is critical for syncytiotrophoblast differentiation and placental developmentSemantic proximity Read ↗

A novel PIGN mutation and prenatal diagnosis of inherited glycosylphosphatidylinositol deficiencySemantic proximity Read ↗

Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored ProteinsSemantic proximity Read ↗

A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model.Semantic proximity Read ↗

A novel intellectual disability syndrome caused by GPI anchor deficiency due to homozygous mutations in PIGT.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.