Article
Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap.
Nature biotechnology - 1 Jul 2025
Gu Jiacheng, Iyer Abhishek, Wesley Ben, Taglialatela Angelo, Leuzzi Giuseppe, Hangai Sho, Decker Aubrianna, Gu Ruoyu, Klickstein Naomi, Shuai Yuanlong, Jankovic Kristina, Parker-Burns Lucy, Jin Yinuo, Zhang Jia Yi, Hong Justin, Niu Xiang, Costa Jonathon A, Pezet Mikael G, Chou Jacqueline, Chen Cao 'Claire', Paiva Margaret, Snoeck Hans-Willem, Landau Dan A, Azizi Elham, Chan Edmond M, Ciccia Alberto, Gaublomme Jellert T
Abstract excerpt
Unlike sequencing-based methods, which require cell lysis, optical pooled genetic screens enable investigation of spatial phenotypes, including cell morphology, protein subcellular localization, cell-cell interactions and tissue organization, in response to targeted CRISPR perturbations. Here we report a multimodal optical pooled CRISPR screening method, which we call CRISPRmap. CRISPRmap combines in situ CRISPR...
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